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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Sep 19;42(9):113149. doi: 10.1016/j.celrep.2023.113149

Figure 1. Generation of TBEV escape variants from T025 and T028 mAbs.

Figure 1.

(A and D) Passaging strategy for selection of escape variants from T025 (A) and T028 (D).

(B and E) Sensitivity of TBEV escape variants (TBEV-MUT25 and TBEV-MUT28) and the parental strain (TBEV-Hypr) to the selecting mAbs T025 (B) and T028 (E), as determined by a neutralization test. Two independent experiments were performed in octuplicate.

(C and F) Surface model of the TBEV E protein with domains I, II, and III colored in red, yellow, and blue, respectively. The amino acid changes in TBEV-MUT25 (C) and TBEV-MUT28 (F) are shown.

(G) Alignment of the E protein sequences of TBEV-Hypr, both escape variants, and other notable TBFVs. Amino acid changes in the E protein sequence are highlighted in yellow for TBEV-MUT25 and in blue for TBEV-MUT28. OHFV, Omsk hemorrhagic fever virus; KFDV, Kyasanur forest disease virus; POWV, Powassan virus; AHFV, Alkhurma hemorrhagic fever virus; LGTV, Langat virus; LIV, louping ill virus.