KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal anti-EGFP mouse IgG1κ [7.1 and 13.1] (1:10,000) | Roche | Cat# 11814460001; RRID: AB_390913 |
| Monoclonal anti-FLAG® tag mouse IgG1 [M2] (1:5,000) | Sigma-Aldrich | Cat# F3165; RRID: AB_259529 |
| Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) | Cell Signaling Technologies | Cat# 9624S; RRID: AB_331817 |
| Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) | Abcam | Cat# ab92570; RRID: AB_10562142 |
| Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) | Abcam | Cat# ab188291 |
| Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) | United States Biological | Cat# 169073 |
| Polyclonal anti-PGK1 Rabbit IgG (1:10,000) | Antibodies Online Inc. | Cat# ABIN568371; RRID: AB_2924789 |
| StarBright® B700 labeled goat antimouse IgG secondary antibody (1:10,000) | Bio-Rad | Cat# 12004158; RRID: AB_2884948 |
| Alexa Fluor® 488 labeled goat antirabbit IgG secondary (1:10,000) | Invitrogen | Cat# A11034; RRID: AB_2576217 |
| ECL-anti-rabbit HRP IgG (1:10,000) | Cytiva | Cat# NA9340; RRID: AB_772191 |
| Chemicals, peptides, and recombinant proteins | ||
| SYTOX Green | Thermo-Fisher | Cat# S7020 |
| β-estradiol | Sigma-Aldrich | Cat# E8875 |
| 1NM-PP1 | APExBIO | Cat# B1299 |
| Ni-NTA agarose | Qiagen | Cat# 30230 |
| Imidazole | Fisher Scientific | Cat# ICN1020335 |
| Dynabeads Protein G | Fisher Scientific | Cat# 10004D |
| PhosStop | Roche | Cat# 4906845001 |
| Pepstatin | Roche | Cat# 11359053001 |
| cOmplete Protease Inhibitor Cocktail | Roche | Cat# 11873580001 |
| PMSF | Sigma-Aldrich | Cat# 78830 |
| Bovine Serum Albumin | Sigma-Aldrich | Cat# A2153 |
| 4-20% Criterion™ TGX Stain-Free™ Gel | Bio-Rad | Cat# 5678095 |
| SurePAGE™ 4–20% Bis-Tris Gel | GenScript | Cat# M00657 |
| Anti-FLAG® M2 Affinity Gel | Sigma-Aldrich | Cat# A2220 |
| 3 × FLAG peptides | Sigma-Aldrich | Cat# F4799 |
| λ-Phosphatase | NEB | Cat# P0753S |
| N6-PhET-ATPγS | Axxora | Cat# BLG-P026-05 |
| p-Nitrobenzyl mesylate (PNBM) | Abcam | Cat# Ab138910 |
| Zymolyse (20T) | AMS Bio | Cat# 120491-1 |
| Protein Kinase A Catalytic Subunit from bovine heart | Sigma-Aldrich | Cat# P2645 |
| Rim4-6His | This paper | NA |
| Rim4-GFP-6His | This paper | NA |
| Rim4(5C)-6His | This paper | NA |
| Rim4(PxLm)-6His | This paper | NA |
| Bmh1-GFP-6His | This paper | NA |
| Bmh2-GFP-6His | This paper | NA |
| Bmh1-3FLAG-6His | This paper | NA |
| 6His-3FLAG-Cdc14 | (Feng et al.)45 | NA |
| Critical commercial assays | ||
| Yeast Transformation Kit | Sigma-Aldrich | Cat# YEAST1 |
| GenCatch™ Plasmid DNA Mini-Prep Kit | Epoch | Cat# 2160250 |
| GenCatch™ Gel Extraction Kit | Epoch | Cat# 2260250 |
| Experimental models: Organisms/strains | ||
| This paper | Table S1 | |
| Recombinant DNA | ||
| This paper | Table S2 | |
| Software and algorithms | ||
| Image Lab™ (version 6.1) | Bio-Rad | https://www.bio-rad.com/en-us/product/image-lab-software |
| ImageJ (version 1.53u) | National Institutes of Health (Public Domain) | https://imagej-nih-gov.foyer.swmed.edu/ij/ |
| SlideBook 6 | Intelligent imaging | https://www.intelligent-imaging.com/slidebook |
| Flowing Software (version 2.5.1) | Turku Bioscience Center | https://bioscience.fi/services/cell-imaging/flowing-software/ |
| SnapGene (version 7.0.2) | Dotmatic | https://www.snapgene.com/ |
| GraphPad Prism (version 10.0.1) | Dotmatic | https://www.graphpad.com/ |
| AlphaFold | DeepMind Technologies | https://alphafold.com/ |
| Robetta | Baker Lab, HHMI’s Janelia Research Campus | http://www.robetta.org/ |