Figure 2. Overexpression of BCL7C, KANSL2, and SIRT2 reduces reactivation of latent HIV in Jurkat and primary cells.
(A–D) J-Lat A2 cells nucleofected with plasmids overexpressing indicated proteins were treated with either DMSO or 10 nM PMA and subjected to FACS for percentage of GFP+ cells (A–C) and western blot for indicated proteins (D). Representative FACS plots in the DMSO-treated group is shown in (A), and quantifications of the percentage of GFP+ cells are shown in (B) and (C).
(E) CD4 T cells from 10 individuals living with HIV on ART were nucleofected with plasmid vectors overexpressing indicated proteins and treated with either DMSO or 50 ng/mL (81 nM) PMA and 1 μM ionomycin. HIV-1 RNAs in the cells were quantified with qRT-PCR. The qPCR signal from each drug combination was normalized to the DMSO group for each individual to calculate the fold difference.
Error bars represent mean ± SD from three experimental replicates (B and C) or mean ± SEM from 10 donors’ results (E). *p < 0.05, **p < 0.01, and ***p < 0.001 by two-tailed Student’s t test.