Figure 4.

Effect of control or Notch3 mutant ASO 18 on Notch3^6691-TAATGA mRNA expression in calvarial osteoblast-enriched (A and B) and bone marrow stromal (C) cells from Notch3^em1Ecan mice. In Panel A, Notch3^6691-TAATGA mRNA levels were obtained 72 h after the addition of Notch3 mutant or control ASO at the indicated doses. In Panel B, Notch1, Notch2, Notch3, Notch3^6691-TAATGA and Notch4 mRNA were detected 72 h after the addition of Notch3 mutant or control ASO at 20μM. In Panel C, Notch3 and Notch3^6691TAATGA mRNA levels were obtained after the addition of Notch 3 mutant (closed circles grey bars,) or control ASO (open circles, white bars,) at 20μM for 72 h. Transcript levels are expressed as copy number following correction for Rpl38. In Panels A and B, values are means ± SD. In Panel C, individual values are shown, and bars and ranges represent mean ± SD; n = 3 or 4 technical replicates. *Significantly different between Notch3 mutant ASO and control ASO by ANOVA with post-hoc analysis by Holm Šidák (Panels A and C) or unpaired t-test (Panel B), p <0.05.