Figure 6.

Cortical bone microarchitecture assessed by μCT of the mid-diaphyseal femur from 2 month old Notch3^em1Ecan mutant male mice and sex-matched littermate controls treated with Notch3 mutant ASO 18 (closed circles, grey bars; n = 32 for wild type mice, n = 26 for Notch3^em1Ecan) or control ASO (open circles, white bars; n = 34 for wild type mice, n = 23 for Notch3^em1Ecan) both at 50 mg/Kg subcutaneously, once a week for 4 weeks prior to sacrifice. Parameters shown are cortical bone volume/total volume (BV/TV, %), cortical porosity (%), cortical thickness (mm) and polar moment of inertia (pMOI). Individual values are shown, and bars and ranges represent means ± SD of biological replicates. *Significantly different between Notch3 mutant and control ASO, p < 0.05. #Significantly different between Notch3^em1Ecan and control, p < 0.05, by ANOVA with post-hoc analysis by Holm Šidák. Representative images show cortical bone osteopenia in Notch3^em1Ecan mutant mice and its amelioration by Notch3 mutant ASOs. Scale bars in the right corner represent 100 μm. Arrow on scout μCT images point to the area analyzed.