| Antibodies |
| Rabbit polyclonal anti-aPKC |
Santa Cruz Biotechnology |
Lot#: G2304 |
| Mouse monoclonal anti-Crb |
DSHB |
Cq4 |
| Mouse monoclonal anti-Myc |
Cell Signaling |
Product #2276 |
| Mouse monoclonal anti-FLAG |
Sigma Aldrich |
Cat# F1804; RRID:AB_262044 |
| Chemicals, peptides, and recombinant proteins |
| 1-NA-PP1 |
Cayman |
Item # 10954 |
| Jasplakinolide |
Cayman |
Item # 11705 |
| Janelia Fluor® Halo Tag® Ligand |
Promega |
Cat# GA1120 |
| Experimental models: Cell lines |
| Hamster: CHO cells |
ATCC |
CRL-11268 |
|
D. melanogaster: Cell line S2: S2-DRSC |
Laboratory of Peter Cherbas |
RRID:CVCL_TZ72 |
| Experimental models: Organisms/strains |
|
D. melanogaster: RNAi of Sxl: y[1] sc[*] v[1]; P{TRiP.HMS00609}attP2 |
Bloomington Drosophila Stock Center |
BDSC:34393; FlyBase: FBtp0064874 |
|
D. melanogaster: Kib::GFP |
Su et al.27
|
|
|
D. melanogaster: UAS-LifeAct-Halo2 |
Bloomington Drosophila Stock Center |
BDSC:67625; FlyBase: FBti0186932 |
|
D. melanogaster: Ubi>Kib-Halo |
This paper |
N/A |
|
D. melanogaster: sGMCA |
Kiehart et al.79
|
N/A |
|
D. melanogaster: Sqh-GFP |
Bloomington Drosophila Stock Center |
BDSC:57145; FlyBase: FBti0150058 |
|
D. melanogaster: Ecad::GFP |
Huang et al.80
|
N/A |
|
D. melanogaster: Ubi>Kib-GFP |
Tokamov et al.8
|
N/A |
|
D. melanogaster: Ubi>Kib𝚫aPKC-GFP |
This paper |
N/A |
|
D. melanogaster: Zip-GFP |
Rauskolb et al.18
|
N/A |
|
D. melanogaster: UAS-SqhDD
|
Mitonaka et al.41
|
N/A |
|
D. melanogaster: Ecad-3XmKate2 |
Pinheiro et al.81
|
N/A |
|
D. melanogaster: Ex-YFP |
Su et al.27
|
N/A |
|
D. melanogaster: Ubi-Mer-Halo |
This paper |
N/A |
|
D. melanogaster: Hpo-YFP |
Su et al.27
|
N/A |
|
D. melanogaster: Halo-Snap-aPKC |
Erdmann et al.82
|
N/A |
|
D. melanogaster: Mer-YFP |
Su et al.27
|
N/A |
|
D. melanogaster: RNAi of Crb: GD14463 |
Vienna Drosophila RNAi Center |
VDRC: 39177 |
|
D. melanogaster: RNAi of Sqh: GD1695 |
Vienna Drosophila RNAi Center |
VDRC: 7916 |
|
D. melanogaster: RNAi of Patj: y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01489}attP2 |
Bloomington Drosophila Stock Center |
BDSC: 35747; FlyBase: FBti0145012 |
|
D. melanogaster: RNAi of Sdt: y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00851}attP2 |
Bloomington Drosophila Stock Center |
BDSC: 33909; FlyBase: FBti0140562 |
|
D. melanogaster: RNAi of Ex: KK100573
|
Vienna Drosophila RNAi Center |
VDRC: 109281 |
|
D. melanogaster: UAS-Utrophin-ABD-GFP |
Rauzi et al.83
|
N/A |
|
D. melanogaster: aPKCas4
|
Hannaford et al.49
|
N/A |
| Recombinant DNA |
| pMT-Kib-GFP |
This paper |
N/A |
| pMT-Par-6-Myc |
This paper |
N/A |
| pMT-aPKC |
This paper |
N/A |
| pAc.5.1-Crbi-3xFLAG |
This paper |
N/A |
| pMT-Ed:GFP |
Johnston et al.47
|
N/A |
| pMT-Ed:GFP:aPKC |
Johnston et al.47
|
N/A |
| Ubi>Kib-GFP-FLAG |
This paper |
N/A |
| Ubi>Kib𝚫aPKC-GFP-FLAG |
This paper |
N/A |
| Ubi>Kib858–1288-GFP-FLAG |
This paper |
N/A |
| Software and algorithms |
| Fiji (ImageJ) |
Schneider et al.84
|
https://imagej.nih.gov/ij/
|
| Cellpose |
Stringer et al.76
|
https://www.cellpose.org
|
| Scikit |
van der Walt et al.75
|
https://scikit-image.org
|
| GraphPad Prism |
GraphPad Software |
N/A |
