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. Author manuscript; available in PMC: 2024 Oct 12.
Published in final edited form as: J Med Chem. 2023 Oct 2;66(19):13821–13837. doi: 10.1021/acs.jmedchem.3c01345

Table 2.

Primers for zCD2 Mutagenesis

Primer: Sequence: Annealing Temperature:
Y745F Fwd 5’-GGAAGGCGGTTTTAACCTGACCA-3’ 63 °C
Y745F Rev 5’-AGGATAATCAGCACACGAC-3’
H573A Fwd 5’-TCCGCCGGGCGCGCACGCAGAAAAAGATACCGC-3’ 72 °C
H574A Rev 5’-CGCACGATGGCCACCGCA-3’