Figure 2.
Metabolic partitioning of maximal respiration. (A) F0 ovaries (1 ovary/female, 4–5 females/tank, 3 tanks/treatment group). (B) 24 hpf F1 embryos (2 embryos/well, 5–6 wells/tank, 3 tanks/treatment group) (data from FCCP plates). Different letters denote statistical differences across treatments within a given parameter (Two-way ANOVA with treatment and tank factors; Fisher’s LSD treatment effects; P<0.05). For Figures 2A and 2B, total maximal respiration is represented by the entire bar (red+green+green/pink hashed sections), and statistical significance (when significant) is denoted by n and o. Maximal mitochondrial respiration is represented by the entire green section, including the portion of the green bar which has pink crossed hashes. The portion of maximal mitochondrial respiration that reflects reserve capacity is cross hashed in pink, and significance values (when significant) are displayed within this segment, denoted by p and r. Non-mitochondrial respiration is represented in red, and significance values (when significant) are displayed within this segment, denoted by c and d.