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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Cytopathology. 2023 Sep 15;34(6):551–561. doi: 10.1111/cyt.13304

Table 2.

Whole transcriptome RNA sequencing. Concordance analysis of each gene expression signature (SETER/PR and PI3Kges) measured from cytospin slides versus fresh frozen RNAlater sample at each time point (day). Abbreviations: N, number of cases; CCC, concordance correlation coefficient; LCI, lower confidence interval; UCI, upper confidence interval; CS_C, cytospin slides fixed in Carnoy’s solution; CS_E, cystopin slides fixed in 95% ethanol.

Signature Duration (days) Method N CCC 95% LCI 95% UCI Rho Bias
PI3Kges 0 CS_C 10 0.96 0.87 0.99 0.97 0.99
CS_E 9 0.85 0.55 0.95 0.94 0.90
1 CS_C 10 0.96 0.85 0.99 0.97 0.99
CS_E 10 0.77 0.44 0.92 0.89 0.87
4 CS_C 10 0.93 0.75 0.98 0.93 1.00
CS_E 10 0.83 0.50 0.95 0.88 0.95
7 CS_C 10 0.93 0.76 0.98 0.95 0.98
CS_E 10 0.64 0.14 0.88 0.71 0.91
SET ER/PR 0 CS_C 10 0.99 0.96 1.00 0.99 1.00
CS_E 9 0.97 0.88 0.99 0.98 0.99
1 CS_C 10 0.99 0.95 1.00 0.99 1.00
CS_E 10 0.96 0.85 0.99 0.97 0.98
4 CS_C 10 0.94 0.79 0.98 0.97 0.97
CS_E 10 0.99 0.95 1.00 0.99 0.99
7 CS_C 10 0.84 0.60 0.94 0.97 0.87
CS_E 10 0.92 0.74 0.98 0.95 0.97