Figure 7. T-bet expression in response to IFNγ signaling is required for pre-MEM and lung-BRM differentiation after influenza infection.

(A) Volcano plot highlighting DEGs in WT vs. IfngR1^−/− GC B cells from day 12 PR8-infected WT/IfngR1^−/− BM chimeras. Three replicates for each cell type were obtained from three independent experiments. (B) T-bet expression in WT and IfngR1^−/− GC B cells from day 10 PR8-infected WT/IfngR1^−/− BM chimeras. Data are representative of three independent experiments. (C) T-bet expression in GC B cells from PR8-infected B6 mice at the indicated time points. Data are representative of three independent experiments (n=5-7 mice). (D) T-bet expression in CXCR3^hi and CXCR3^lo pre-MEMs from day 10 PR8-infected B6 mice. Data are representative of three independent experiments (n=5-6 mice). P values were determined by one-way ANOVA with a post-hoc Kruskal–Wallis comparison test. (E-F) B6 and Tbx21^−/− mice were infected with PR8 and GC B cells from the med-LN were analyzed on day 10. (E) Frequency of pre-MEMs. (F) Number of CXCR3⁺ and CXCR3⁻ pre-MEMs. Data are representative of two independent experiments (n=5mice). P values were determined using a two-tailed Student’s t-test. (G and H) WT/Tbx21^−/− BM chimeras were infected with PR8. (G) Frequency of NP-specific BRMs within the B6 and Tbx21^−/− compartments in the lungs on day 30. (H) Ratio of B6 to Tbx21^−/− naïve B cells and NP-specific BRMs. Data are representative of three independent experiments (n=5-6 mice). P values were determined using a two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.