FIG. 3.
Mapping of the GM193663r mutations on the primary structure of EF2. (A) Overlapping fragments of the EFT2 gene used in the mapping by SSCP. Base pair 1 corresponds to the A in the first ATG codon. (B) Position and nature of the amino acid change in the sequenced mutant alleles of EFT2. (C) Protein sequence comparison of the region in EF2 from different organisms corresponding to the region where most GM193663 resistance mutations were found in S. cerevisiae. The mutated positions are boxed, and the amino acid residue found in the resistant mutant is shown on top of each box. Fu, fungi; An, animals; Pl, plants; Pr, protists.