TABLE 2.
Specific binding of [3H]sordarin to macromolecules in cell extracts from wild-type and GM193663r mutant strainsa
| Strain | Mutation | dpmb
|
|
|---|---|---|---|
| Expt 1 | Expt 2 | ||
| 373 | None | 85,640 | 71,265 |
| FPR1-3 | Ala562Pro | 125 | 0 |
| FPR1-5 | Phe612Leu | 172 | 0 |
| SEY6210 | None | 23,184 | 188,515 |
| FPR1-18 | Ile529Ser | 229 | 76 |
| FPR1-19 | Ser523Thr | 0 | 191 |
| FPR1-21 | Tyr521Asp | 0 | 87 |
| FPR1-24 | Val561Phe | 4,853 | 1,040 |
| FPR1-26 | Ala475Val | 0 | 1,770 |
| FPR1-30 | Pro559Ser | 99 | 7,053 |
a
Resistant isolates are listed below their corresponding parental strain.
b
3H radioactivity associated with macromolecules, measured in two independent experiments (experiments 1 and 2), as described in Materials and Methods.