| Antibodies |
| Anti-BMAL1 |
Abcam |
ab93806; RRID: AB_10675117 |
| Anti-p84 |
Genetex |
GTX70220; RRID: AB_372637 |
| Anti-RFP |
Rockland |
600–401-379 |
| Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) |
Abcam |
ab150079 |
| Anti-Tubulin |
Sigma |
T6199 |
| Chemicals, peptides, and recombinant proteins |
| U-13C6 glucose |
Cambridge Isotope Laboratories |
CLM-1396-PK |
| Critical commercial assays |
| Ultra Sensitive Mouse Insulin ELISA Kit |
Crystal Chem |
90080 |
| Deposited data |
| RNA sequencing – WT and double RE liver and muscle |
This paper |
GSE197455 |
| RNA sequencing – WT, Bmal1 KO, and Muscle-RE muscle |
This paper |
GSE197726 |
| RNA sequencing – Night feeding dataset, all genotypes, liver and muscle |
This paper |
GSE228389 |
| Experimental models: Organisms/strains |
| Mouse: Wild Type (WT) – 1.Bmal1wt/wt, Alfp-cretg/0; 2. Bmal1wt/wt, Hsa-cretg/0; 3. Bmal1wt/wt, Alfp-cretg/0, Hsa- cretg/0 |
S.A. Benitah lab, P.S. Welz, This study |
|
| Mouse: Bmal1 knockout (KO) – Bmal1stopFL/stopFL
|
S.A. Benitah lab, P.S. Welz, This study |
|
| Mouse: Bmal1 hepatocyte-reconstituted (Liver- RE) – Bmal1stopFL/stopFL; Alfp-cretg/0
|
S.A. Benitah lab, P.S. Welz, This study |
|
| Mouse: Bmal1 skeletal muscle-reconstituted (Muscle- RE) – Bmal1stopFL/stopFL; Hsa-cretg/0
|
S.A. Benitah lab, P.S. Welz, This study |
|
| Mouse: Bmal1 hepatocyte + skeletal muscle- reconstituted (Liver+Muscle-RE) – Bmal1stopFL/stopFL; Alfp- cretg/0; Hsa-cretg/0
|
S.A. Benitah lab, P.S. Welz, This study |
|
| Oligonucleotides |
| BMAL1 For - GCAGTGCCACTGACTACCAAGA |
This study |
|
| BMAL1Rev - TCCTGGACATTGCATTGCAT |
This study |
|
| Rn18S For - CGCCGCTAGAGGTGAAATTC |
This study |
|
| Rn18S Rev - CGAACCTCCGACTTTCGTTCT |
This study |
|
| Software and algorithms |
|
dryR
|
Weger et al., 2021, PNAS
|
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| JTK_CYCLE |
Hughes et al., 2010, J. Biol. Rhythms.
|
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| DAVID Bioinformatics Resource |
Huang, Sherman and Lempicki, 2009, Nat. Protoc.
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| Nitecap |
Brooks et al., 2022, J. Biol. Rhythms.
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