Figure 3. LEC S1pr1 deficiency promotes CD4 T cell infiltration following lymphatic surgery.

(A) Flow cytometric gating scheme for determining tail skin immune cell populations. Flow cytometric analysis was performed d21 after lymphatic surgery. (B-G) Quantification of CD45⁺ cells (B), CD8⁺ T cells, (C), CD4⁺ T cells (D), CD4⁺IFN-ɣ⁺ Th1 cells (E), CD4⁺IL-4⁺ Th2 cells (F), and Foxp3⁺CD25⁺CD4⁺ Treg cells (G) in tail skin (n ≥ 4 per each group). (H) Representative IF staining of CD4 (red) and LYVE1 (green) of the lymphedema mouse tail skin from WT or S1pr1^LECKO mice. DAPI (blue) stains for the nucleus. Arrows indicate CD4 T cells surrounding lymphatic vessels. Scale bar = 50 μm. (I) Quantification of the CD4 T cell staining presented in H (n ≥ 5 per each group). Data are presented as mean ± SEM; * p < 0.05, ** p < 0.01, and ns (not significant) by the Mann-Whitney test.