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. Author manuscript; available in PMC: 2024 Apr 13.
Published in final edited form as: Clin Cancer Res. 2023 Oct 13;29(20):4242–4255. doi: 10.1158/1078-0432.CCR-23-0653

Figure 4. CD3+CD4+CD38+CD39+ cells found in the melanoma tumor microenvironment are associated with Ki67- CD8+ T-cells.

Figure 4.

Baseline tumor tissue FFPE samples from metastatic melanoma patients treated with checkpoint inhibition were evaluated by multiplex immunofluorescent imaging. (A) Representative images of CD3 (white), CD38 (green), DAPI (blue), SOX10/100 (cyan), CD4 (purple), CD39 (orange), CD8 (red), and Ki67 (pink) staining, along with the merged image are shown. (B) Cells expressing the marker set CD3+CD4+CD38+CD39+ are artificially colored purple, CD3+CD8+Ki67- cells colored orange and CD3+CD8+Ki67+ colored blue. CD3+CD4+CD38+CD39+ and CD3+CD8+ cells in contact with one another are filled in by color and those not in contact are only outlined. (C) The frequency of Ki67- and Ki67+ CD3+CD8+ cells in each specimen are plotted. Each dot pair represents one patient. (D) The frequency of CD3+CD8+Ki67- and CD3+CD8+Ki67+ adjacent to CD3+CD4+CD38+CD39+ cells as a percent of the parent Ki67−/+ populations are plotted. Each dot pair represents one patient. The corresponding p-value was determined by a paired t-test.