KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti Syt1 | Synaptic Systems | Cat# 105-011C2: RRID:AB_2619760 |
| Chicken polyclonal anti MAP2 | Encor | CPCA-MAP2, RRID:AB_2138173 |
| Rabbit polyclonal anti Syn1 | Sudhof Lab | Clone YZ6078, RRID: AB_2861225 |
| Mouse monoclonal anti PSD95 | Thermo | Cat# MA146: RRID:AB_2092361 |
| Rabbit polyclonal anti APP | Ho et al., 2008 | Clone U955 |
| Mouse monoclonal anti APP | Sigma | Cat# MABN380: RRID: AB_2714163 |
| Rabbit polyclonal anti CASK | Neuromab | Cat# O14936: RRID: AB_2877188 |
| Rabbit polyclonal anti Fe65 | Cao and Südhof, 2001 | Clone 4194 |
| Rabbit polyclonal anti Mint1 | Ho et al., 2008 | Clone P730 |
| Rabbit polyclonal anti Nlgn1 | Jahn Monoclonals | Clone 4C12 |
| Rabbit polyclonal anti pan-Nrxn | Sclip and Südhof, 2020 | Clone G394, RRID: AB_2800397 |
| Rabbit polyclonal anti RIM1/2 | Acuna et al., 2015 | Clone U952 |
| Rabbit polyclonal anti Stx1 a/b | Zhou et al., 2012 | Clone 438B |
| Rabbit polyclonal anti PSD95 | Patzke et al., 2019 | Clone L667: RRID:AB_2800396 |
| Mouse monoclonal anti L1CAM | Sigma | Cat# L4543: RRID:AB_609903 |
| Rabbit monoclonal anti APP | Abcam | Cat# Ab32136: RRID: AB_2289606 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Accutase | Innovative Cell | Cat# AT104 |
| Gem21 Neuroplex supplement | Gemini Bio | Cat# 400–160 |
| BDNF | PeproTech | Cat# 450–02 |
| Doxycycline | Sigma-Aldrich | Cat# D9891 |
| DMEM media | Thermo Fisher | Cat# 11965092 |
| DMEM/F-12 media | Thermo Fisher | Cat# 11320082 |
| HyClone fetal bovine serum (FBS) | GE Healthcare | Cat# SH30071.03 |
| MEM media | Thermo Fisher | Cat# 51200038 |
| Matrigel | BD Biosciences | Cat# 356230 |
| mTeSR1 media | Stem Cell Technologies | Cat# 85850 |
| N-2 supplement | Thermo Fisher | Cat# 17502048 |
| Neurobasal-A Medium | Thermo Fisher | Cat# 10888022 |
| NT-3 | PeproTech | Cat# 450–03 |
| Puromycin | Sigma-Aldrich | Cat# P8833 |
| Thiazovivin | Bio Vision | Cat# 1681 |
| Laminin-1 | Invitrogen | Cat# 23017–015 |
| Trypsin / EDTA | Thermo Fisher | Cat# 25300120 |
| Papain, Suspension | Worthington Biochemical | Cat# LS003127 |
| Cytosine arabinofuranoside (AraC) | Sigma-Aldrich | Cat# C6645 |
| Glutamax | Thermo Fisher | Cat# 35050079 |
| TTX | Fisher Scientific | Cat# 50-753-2807 |
| EDTA | Sigma-Aldrich | Cat# E6758-100G |
| Non-Essential Amino Acids | Thermo Fisher | Cat# 11140076 |
| Human Holotransferrin | Gemini Bio | Cat# 800-131P |
| Total Human Abeta ELISA | Clonetech | Cat# 27729A |
| DMSO, Cell Culture Grade | Sigma | Cat# D2650 |
| DAPT | Tocris | Cat# 2634 |
| LY411575 | Selleckchem | Cat# S2714 |
| Rosuvastatin | Selleckchem | Cat# S2169 |
| Simvastatin | Selleckchem | Cat# S1792 |
| TRIzol | Invitrogen | Cat# 15596026 |
| RNeasy Plus Mini Kit | Qiagen | Cat# 74104 |
| Filipin | Sigma | Cat# SAE0088 |
| Amplex Red Cholesterol Kit | Invitrogen | Cat# A12216 |
| Experimental Models: Cell Lines | ||
| Human H1-ESCs | WiCell Research Institute | Cat# WA01 |
| HEK293T cells | ATCC | Cat# CRL-11268 |
| Experimental Models: Organisms/Strains | ||
| CD-1 IGS mouse | Charles River | Cat# Crl:CD1(ICR) |
| Quantitative qRT-PCR | ||
| Human ACAT2 | IDT | Hs.PT.56a.26153626 |
| Human ACTB | IDT | Hs.PT.39a.22214847 |
| Human DBH | IDT | Hs.PT.58.39251730 |
| Human DHCR24 | IDT | Hs.PT.58.4561516 |
| Human DHCR7 | IDT | Hs.PT.58.38763048 |
| Human DUSP4 | IDT | Hs.PT.58.1640850 |
| Human DUSP5 | IDT | Hs.PT.58.1558918 |
| Human EGR1 | IDT | Hs.PT.58.40805543.g |
| Human FDFT1 | IDT | Hs.PT.58.25526506 |
| Human FOS | IDT | Hs.PT.58.15540029 |
| Human HMGCR | IDT | Hs.PT.58.41105492 |
| Human HMGCS1 | IDT | Hs.PT.58.4084870 |
| Human IDI1 | IDT | Hs.PT.58.19303620 |
| Human JUNB | IDT | Hs.PT.58.27174746.g |
| Human LDHA | IDT | Hs.PT.58.22929122 |
| Human LDLR | IDT | Hs.PT.58.2004261 |
| Human LSS | IDT | Hs.PT.58.24976485 |
| Human MAP2 | IDT | Hs.PT.58.20680759 |
| Human MVD | IDT | Hs.PT.58.39035173 |
| Human NAB2 | IDT | Hs.PT.58.24622150 |
| Human NR4A1 | IDT | Hs.PT.58.20779713 |
| Human SCD | IDT | Hs.PT.58.45714389 |
| Human SREBF2 | IDT | Hs.PT.58.45335433 |
| Human SV2C | IDT | Hs.PT.58.4640250 |
| Human TH | IDT | Hs.PT.58.369742 |
| Human TMEM97 | IDT | Hs.PT.58.40917738 |
| Human VEGFA | IDT | Hs.PT.58.1149801 |
| Human VGF | IDT | Hs.PT.58.26072166 |
| Recombinant DNA | ||
| FUW-TetO-Ng2-T2A-puromycin | Zhang et al., 2013 | 52047 Addgene (lentiviral vector) |
| FUW-rtTA | Zhang et al., 2013 | 20342 Addgene (lentiviral vector) |
| FUW-TetO-EGFP | Vierbuchen et al., 2010 | 30130 Addgene (lentiviral vector) |
| pREV | Dull et al., 1998 | 12253 Addgene (lentiviral helper vector) |
| pRRE | Dull et al., 1998 | 12251 Addgene (lentiviral helper vector) |
| pVSVg | Patzke et al., 2019 | 12259 Addgene (lentiviral helper vector) |
| Deposited Datasets | ||
| Bulk RNA-seq data | GEO | GSE206102 |
| Software and Algorithms | ||
| ImageJ | NIH | RRID: SCR_003070 |
| ImageStudio software | Li-Cor | RRID:SCR_013715 |
| pClamp | Molecular Devices | RRID:SCR_011323 |
| Nikon NIS-Elements | Nikon | RRID:SCR_014329 |
| GraphPad Prism | GraphPad Prism | RRID:SCR_002798 |
| RStudio | RStudio Team | RRID:SCR_000432 |
| DESeq2 | Bioconductor (Love et al., 2014) | RRID:SCR_015687 |