| Antibodies |
|
| Puromycin |
Millipore Sigma |
Catalog #: MABE343; RRID:AB_2566826 |
| α-Tubulin |
Cell Signaling Technology |
Catalog #: 3873; RRID:AB_1904178 |
| p-p70 S6K |
Cell Signaling Technology |
Catalog #: 9234; RRID:AB_2269803 |
| p-4E-BP1 |
Cell Signaling Technology |
Catalog #: 2855; RRID:AB_560835 |
| p70 S6K |
Cell Signaling Technology |
Catalog #: 2708; RRID:AB_390722 |
| 4E-BP1 |
Cell Signaling Technology |
Catalog #: 9644; RRID:AB_2097841 |
| p-eIF2α |
Cell Signaling Technology |
Catalog #: 9721; RRID:AB_330951 |
| eIF2α |
Cell Signaling Technology |
Catalog #: 9722; RRID:AB_2230924 |
| ATF-4 |
Cell Signaling Technology |
Catalog #: 11815; RRID:AB_2616025 |
| EEF1A1 |
Cell Signaling Technology |
Catalog #: 3586; RRID:AB_2096963 |
| RAE1 |
Abcam |
Catalog #: ab36139; RRID:AB_2175345 |
| HARS |
Abcam |
Catalog #: ab137591 |
| V5 |
Fisher Scientific |
Catalog #: R960CUS; RRID:AB_2792973 |
| HDAC1 |
Cell Signaling Technology |
Catalog #: 5356; RRID:AB_10612242 |
| HDAC2 |
Cell Signaling Technology |
Catalog #: 5113; RRID:AB_10624871 |
| HDAC3 |
Cell Signaling Technology |
Catalog #: 3949; RRID:AB_2118371 |
| RbAp46 (OTI5A4) |
Novus Biologicals |
Catalog #: NBP2–01308; RRID:AB_2725199 |
| RbAp48 (13D10) |
Novus Biologicals |
Catalog #: NBP1–41201; RRID:AB_2238239 |
| CHD4 |
Abcam |
Catalog #: ab70469; RRID:AB_2229454 |
| MBD3 |
Abcam |
Catalog #: ab157464 |
| MTA1 |
Abcam |
Catalog #: ab71153; RRID:AB_1269482 |
| P66a |
Novus Biologicals |
Catalog #: NB100-56643; RRID:AB_2294492 |
| LSD1 |
Abcam |
Catalog #: ab17721; RRID:AB_443964 |
| TRIM28 |
Abcam |
Catalog #: ab10483; RRID:AB_297222 |
| Caspase 3 |
Cell Signaling Technology |
Catalog #: 9662; RRID:AB_331439 |
| Cleaved caspase 3 |
Cell Signaling Technology |
Catalog #: 9661; RRID:AB_2341188 |
| PARP |
Cell Signaling Technology |
Catalog #: 9532; RRID:AB_659884 |
| SOD1 |
Abcam |
Catalog #: ab13498; RRID:AB_300402 |
| Catalase |
Abcam |
Catalog #: ab16731; RRID:AB_302482 |
| Thioredoxin |
Abcam |
Catalog #: ab26320; RRID:AB_778412 |
| anti-mouse HRP-conjugated |
Cell Signaling Technology |
Catalog #: 7076; RRID:AB_330924 |
| anti-rabbit HRP-conjugated |
Cell Signaling Technology |
Catalog #: 7074; RRID:AB_2099233 |
| IgG |
Cell Signaling Technology |
Catalog #: 5415; RRID:AB_10829607 |
| H3K27Ac |
Abcam |
Catalog #: ab4729; RRID:AB_2118291 |
| HDAC1 |
Abcam |
Catalog #: ab7028; RRID:AB_305705 |
| TyrRS Antibody (H-11) |
Santa Cruz Biotechnology |
Catalog #: sc-166741; RRID:AB_2219253 |
| Beta Actin |
Cell Signaling Technology |
Catalog #: 4967; RRID:AB_330288 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Homoharringtonine |
Millipore Sigma |
Catalog #: SML1091 |
| Homoharringtonine |
Cayman Chemical |
Catalog #: 14631 |
| Trichostatin A |
Millipore Sigma |
Catalog #: T1952 |
| Cycloheximide |
Fisher Scientific |
Catalog #: AAJ6690103 |
| Torin-1 |
Fisher Scientific |
Catalog #: NC0418592 |
| Hydrogen peroxide |
Millipore Sigma |
Catalog#: H1009 |
| Tunicamycin |
Cayman Chemical |
Catalog#: 11445 |
| Trizol |
Life Technologies |
Catalog #: 15596026 |
| SYBR Select Master Mix |
Applied Biosystems |
Catalog #: 4472908 |
| Puromycin |
Sigma Aldrich |
Catalog #: P9620 |
|
| Critical commercial assays |
|
| ChIP-IT Express Enzymatic kit |
Active Motif |
Catalog #: 53009 |
| NE-PER Nuclear and Cytoplasmic Extraction Reagents |
Thermo Scientific |
Catalog #: 78833 |
| Silver Stain Plus Kit |
BIO-RAD |
Catalog#: 1610449 |
| Cell Counting Kit-8 |
Dojindo |
Catalog #: CK04–01 |
| CM-H2DCFDA |
Invitrogen |
Catalog #: C6827 |
|
| Deposited data |
|
| CHIP-Seq data |
This paper |
GEO: GSE155309
|
|
| Experimental models: Cell lines |
|
| HEK-293 |
ATCC |
CRL-1573 |
| HEK-293T |
ATCC |
CRL-3216 |
| Primary Mouse embryonic fibroblasts |
This paper |
N/A |
| B16-F10 |
ATCC |
CRL-6475 |
|
| Experimental models: Organisms/strains |
|
| Mouse: Yars+/ΔNLS C57BL/6J |
This paper |
N/A |
|
| Recombinant DNA |
|
| shControl |
Santa Cruz Biotechnology |
Catalog #: sc-108060 |
| shE2F1 |
Santa Cruz Biotechnology |
Catalog #: sc-29297-SH |
| InterPlay Mammalian TAP System |
Agilent |
Catalog#: 240101 |
|
| Software and algorithms |
|
| Graphpad Prism v9 |
GraphPad Software, LLC |
https://www.graphpad.com/scientific-software/prism/
|
| ImageJ |
Schneider et al.54
|
https://imagej.nih.gov/ij/
|
| Bowtie2 |
Langmead and Salzberg55
|
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
|
| Samtools |
Li et al.56
|
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
|
| FlowJo |
Becton, Dickinson and Company |
https://www.flowjo.com/solutions/flowjo
|
