(A) Immunostaining of endogenous N-cadherin in cultured hippocampal neurons left unstimulated (CTRL), treated with PTX by itself, or treated with PTX in combination with calpain inhibitors as indicated. Higher magnification views of representative secondary dendrites (boxed regions) are shown below each neuron. Scale, 10 μm. (B) Quantification of images from (A) (p < 0.0001, F=9.159, DF=5) (n=36–40 neurons; one-way ANOVA and Sidak’s post-hoc test, *p<0.05, ****p<0.0001, ns=nonsignificant).