(A-E) Immunostaining of hippocampal neurons for surface GluA2 (sGluA2) (A) left unstimulated (CTRL), or treated with (B) PTX alone, (C) PTX and beta secretase inhibitor (BSI), (D) PTX and ADAM10 inhibitor GI254023X, or (E) PTX and both BSI+GI254023X (combo). Higher magnification views of representative secondary dendrites (boxed regions) are shown below each neuron. Scale, 10 μm. (F) Quantification of images in (A-E) (p< 0.0001, F= 17.29, DF=3) n=14–21; one way ANOVA and Tukey’s multiple comparison test, *p<0.05, **p<0.001, ****p<0.0001, ns=nonsignificant).