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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Thromb Haemost. 2023 Jun 29;21(11):3124–3137. doi: 10.1016/j.jtha.2023.06.021

Figure 1. Relative activities and antigens of secreted Protein C with missense mutations in signal peptide and propeptide.

Figure 1.

The constructs with missense mutations were overexpressed in HEK 293T cells in the medium with 10μM vitamin K, and anticoagulant activity, amidolytic activity, total antigens and γ-carboxylated antigens of the secreted PC were determined by corresponding methods (see methods), respectively. Relative values were normalized by wild-type PC which value is defined as “1”. A. Relative PC’s anticoagulant activity. B. Relative PC’s chromogenic activity. C. Relative total antigen of PC. D. Relative γ-carboxylated PC. The t-test is performed by Graphpad Prism 7. n.s. indicates no significant difference. * indicates p<0.05, and ** indicates p<0.01.