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. Author manuscript; available in PMC: 2024 Oct 5.
Published in final edited form as: Structure. 2023 Aug 24;31(10):1184–1199.e3. doi: 10.1016/j.str.2023.07.015

Figure 2. Paramagnetic NMR relaxation enhancements reveal locations in FP close to nitroxide spin labels deep or shallow within bicelles.

Figure 2.

(A) The NMR-identified helices16,19 are plotted. (B,C) PREs emanating from nitroxide spin-labeled phospholipids at pH 5.0 at 303 K were detected by NMR at 800 MHz. Γ2 values are the differences between rate constants fitted to 1H T2 decays of the paramagnetic and diamagnetic samples. (B) 5-doxyl PC conferred high Γ2 values (large line broadening) to polypeptide groups that it approached. (C) 14-doxyl PC conferred high Γ2 to the most deeply inserted groups. Related to Figs. S1 – S3.