Figure 4. Thinning of both leaflets DMPC bilayer near FP.

The states are (A) Asp⁻¹ 830, (B) Asp⁰ 830, and (C) Asp⁰ 820/830/839/843. The mean distances from the center-of-mass of the choline headgroup to the bilayer mid-plane were monitored every 100 ps, after equilibration with the NMR-derived restraints. The gold and green peaks plot the unperturbed distances remote from FP. The purple and orange peaks represent the mid-plane distances to the headgroups in the cis and trans-leaflets, respectively, perturbed by proximity to FP, i.e., within 20 Å of the Leu828 backbone at the tip of FP. Related to Fig. S15.