Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli BL21-Gold (DE3) RIL	Agilent	230245
Chemicals, peptides, and recombinant proteins
Ni-NTA resin	GenScript	L00250
TEV protease	GenScript	Z03030
DMPC	Avanti Polar Lipids	850345
DH7PC	Avanti Polar Lipids	850306
16:0–5 Doxyl PC	Avanti Polar Lipids	810601
16:0–14 Doxyl PC	Avanti Polar Lipids	810605C discontinued; use 810604
Recombinant FP prepared from GB1-FP	Koppisetti et al. (2021)	N/A
Deposited data
NMR-biased MD trajectories in .DCD format	This paper	DOI 10.17605/OSF.IO/95TRE
collective variables,.psf files, and .pdb files used for MD simulations	This paper	DOI 10.17605/OSF.IO/95TRE
Oligonucleotides
GB1-FP_seq with GGGKKKK tag prior to deletion: gat ata cat atg cac cac cat cac cat cac ggc ggc acg tat aaa ctt att ctc aat ggt aag aca ctt aaa ggg gag act acg aca gag gcg gta gac gca gca acg gca gag aag gtg ttc aaa caa tac gca aat gat aac ggg gtg gac ggg gaa tgg aca tac gat gat gcg acc aaa aca ttt act gtc act gaa ggg ggt gag aat tta tat ttt caa agt ttt att gaa gac ttg ctt ttc aac aaa gtg acg ctg gca gac gcg ggt ttc att aag cag tat ggg gac tgc ttg ggt gat att gca gcg cgg gat ttg att tgt gct caa aag ttc aat ggc ggt ggt ggt aaa aag aaa aaa tga gaa ttc gag ctc	Koppisetti et al. (2021)	N/A
Recombinant DNA
pET27b(+) with GB1-FP insert and deletion of C-terminal GGGKKKK tag. Hosted in E. coli BL21-Gold (DE3) RIL.	Koppisetti et al. (2021)	N/A
Software and algorithms
Tcl analysis scripts and PLUMED-VMD analysis scripts	This paper	DOI 10.17605/OSF.IO/95TRE