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. Author manuscript; available in PMC: 2024 Apr 2.
Published in final edited form as: Mol Cancer Res. 2023 Oct 2;21(10):1010–1016. doi: 10.1158/1541-7786.MCR-23-0049

Figure 3. Mitochondrial uncoupling inhibits reductive carboxylation during anchorage-independent growth.

Figure 3

(A, B) SK-N-BE(2) and NB16 cells in monolayer culture, or spheroid culture condition treated with DMSO or 1 μM NEN for 3 hours, were labeled with U-13C-glutamine for 3 hours. Relative isotopic labeling fractions or ratios of metabolites were measured using LC-MS. (C) Morphology of SK-N-BE(2) and NB16 cells treated with DMSO or 1 μM NEN for 12 days in low attachment dishes. (Scale bar: 500μm) (D) Quantitation of spheroid and cell number from samples shown in (C).