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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Jun 12;42(6):112634. doi: 10.1016/j.celrep.2023.112634

Figure 5. The AhR blockade boosts HIV-1 outgrowth in CD4+ T cells of ART-treated PLWH.

Figure 5.

(A) Shown is the flow chart of the viral outgrowth assay (VOA). Briefly, memory CD4+ T cells isolated from the PBMCs of ART-treated PLWH were activated with CD3/CD28 Abs and cultured with rhIL-2 in the presence/absence of FICZ (100 nM) or CH223191 (10 μM). At day 12 post-infection, HIV-1 replication and cell viability were measured.

(B–E) Shown are the percentages of HIV-p24+ T cells (8 splitting replicates pooled per condition) from one representative donor (ART #2; Table S1) (B), the statistical analysis of the percentage of HIV-p24+ cells (C), the HIV-p24 levels in cell-culture supernatants (median values of HIV-p24 levels from 8 splitting replicates per condition) (D), and the viability of cells harvested at day 12 of VOA (E) in experiments performed with cells from n = 7 donors. Friedman test p values with Dunn’s multiple comparisons indicated on the graphs (*p < 0.05; **p < 0.01; ***p < 0.001).

Shown are boxes and whisker plots, with minimum to maximum values (C–E).