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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 May 23;42(6):112556. doi: 10.1016/j.celrep.2023.112556

Figure 1. Ex vivo CD4+ T cell model to define ISG restriction of HIV.

Figure 1.

(A) Schematic of approach for evaluating ISG function in primary CD4+ T cells.

(B and C) NTC-delivered and IRF9-edited CD4+ T cells were infected with HIV strains Q23.BG505 and LAI, respectively, at an MOI of 0.02. Reverse transcriptase (RT) activity was measured over the course of infection. Left, representative infection curves from one donor from infections with and without IFN treatment (with IFN, no IFN). Right, 6 day post-infection (dpi) RT activity from infections with and without IFN from 10 independent experiments that used 8 unique donors. Dot plots are paired by experiment, and the median IFN sensitivity across experiments is depicted below (IFN sensitivity = untreated day 6 RT/with IFN day 6 RT).

**p < 0.002, paired Wilcoxon rank test. Non-significant (ns) indicates p > 0.05.

See also Figure S1.