| Antibodies |
|
| ChromoTek GFP-Trap agarose beads |
ChromoTek |
Cat# Gta |
| ChromoTek Binding Control Agarose Beads |
ChromoTek |
Cat# bab |
| Rabbit Living Colors dsRed Polyclonal Antibody |
Takara |
Cat# 632496, RRID:AB_10013483 |
| Mouse anti-GFP |
Santa Cruz Biotechnology |
Cat# sc-9996, RRID:AB_627695 |
| Rabbit anti Btbd11 |
This paper |
N/A |
| Rabbit anti Btbd11 |
Abclonal |
Cat# A20192 |
| Mouse anti Psd95 (clone K28/74) |
NeuroMab |
Cat# K28/74, RRID:AB_2877478) |
| Mouse anti GST |
Sigma-Aldrich |
Cat# SAB4200692, RRID:AB_259845 |
| Rabbit anti Parvalbumin |
Swant |
Cat# PV27, RRID:AB_2631173 |
| Mouse anti Alpha-tubulin |
Santa Cruz Biotechnology |
Cat# sc-5286, RRID:AB_628411 |
| Mouse anti Synaptophysin |
Sigma-Aldrich |
Cat# S5768, RRID:AB_477523 |
| Mouse anti Lhx6 |
Santa Cruz Biotechnology |
Cat# sc-271433, RRID:AB_10649856 |
| Chicken anti GFP |
Abcam |
Cat# ab13970, RRID:AB_300798 |
| Mouse anti Psd95 (clone K28/43) |
NeuroMab |
Cat# K28/43, RRID:AB_2877189 |
| Mouse anti GAD67 |
Santa Cruz Biotechnology |
Cat# sc-28376, RRID:AB_627650 |
| Mouse anti parvalbumin |
Swant |
Cat# 235, RRID:AB_10000343 |
| Mouse anti Gephyrin |
Synaptic Systems |
Cat# 147011, RRID:AB_887717 |
| IRDye 680RD Goat anti-Mouse IgG Secondary Antibody |
LI-COR Biosciences |
Cat# 926–68070, RRID:AB_10956588 |
| IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody |
LI-COR Biosciences |
Cat# 926–32211, RRID:AB_621843 |
| IRDye 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody |
LI-COR Biosciences |
Cat# 926–68050, RRID:AB_2783642 |
| IRDye 800CW Goat anti-Mouse IgG2a-Specific Secondary Antibody |
LI-COR Biosciences |
Cat# 926–32351, RRID:AB_2782998 |
| Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 |
Invitrogen |
Cat# A-11011, RRID:AB_143157 |
| Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Invitrogen |
Cat# A-21131, RRID:AB_2535771 |
| Goat anti-Chicken IgY (H + L) Secondary Antibody, Alexa Fluor 488 |
Invitrogen |
Cat# A-11039, RRID:AB_142924 |
| Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 |
Invitrogen |
Cat# A-21134, RRID:AB_2535773 |
| DyLight 405-AffiniPure Goat Anti-Mouse IgG, Fc_ Subclass 3 Specific |
Jackson Immuno Research |
Cat# 115-475-209, RRID:AB_2338803 |
| Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 |
Invitrogen |
Cat# A-21124, RRID:AB_2535766 |
| Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
Invitrogen |
Cat# A-21240, RRID:AB_2535809 |
|
| Bacterial and virus strains |
|
| Escherichia coli: Stbl3 |
ThermoFisher |
Cat #C737303 |
| Escherichia coli: Top10 |
ThermoFisher |
Cat #C404010 |
| PJ69-4A yeast cells |
(James et al., 1996)56
|
N/A |
| AAV.CMV.PI.EGFP.WPRE.bGH (serotype 2/9) |
Addgene |
Cat# 105530-AAV9 |
| AAV.CMV.FII.eGFP-Cre.WPRE.SV40 (serotype 2/9) |
Addgene |
Cat# 105545-AAV9 |
| AAV-FLEX-tdTomato |
Addgene |
Cat# 28306-AAV9 |
| AAV-S5E2-dTom-nlsdTom (serotype 2/9) |
Janelia Farm |
N/A |
| AAV.Syn.NES-jRGECO1a.WPRE.SV40 (serotype 2/9) |
Addgene |
Cat# 100854-AAV9 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Sodium pyrophosphate decahydrate |
Sigma-Aldrich |
Cat# 221368; CAS: 13472-36-1 |
| Sodium orthovanadate |
Sigma-Aldrich |
Cat# S6508; CAS: 13721-39-6 |
| Sodium Fluoride |
Sigma-Aldrich |
Cat# S7920, CAS: 7681-49-4 |
| Sodium Chloride |
Sigma-Aldrich |
Cat# S7653, CAS: 7647-14-5 |
| Potassium Chloride |
Sigma-Aldrich |
Cat# P9333, CAS: 7447-40-7 |
| Sodium deoxycholate |
Sigma-Aldrich |
Cat# 30970, CAS: 302-95-4 |
| Triton X-100 |
Sigma-Aldrich |
Cat# T8787, CAS: 9036-19-5 |
| IGEPAL CA-630 (NP-40) |
Sigma-Aldrich |
Cat# I8896, CAS: 9002-93-1 |
| β-Mercaptoethanol |
Millipore |
Cat# 444203, CAS: 60-24-2 |
| MES |
Fisher |
Cat# 50–188-3347 |
| HEPES, ≥99.5% (titration) |
Sigma-Aldrich |
Cat# H3375; CAS: 7365-45-9 |
| Sucrose |
Sigma-Aldrich |
Cat# S7903; CAS: 57-50-1 |
| cOmplete, EDTA-free |
Roche |
Cat# 04 693 132 001 |
| Pefabloc SC (AEBSF) |
Roche |
Cat# 11427393103 |
| PhosSTOP™
|
Roche |
Cat# 04 906 837 001 |
| Okadaic acid, Free acid, >98% |
LC Laboratories |
Cat# O-2220 CAS: 78111-17-8 |
| Ethylenediaminetetraacetic acid disodium salt dihydrate |
Sigma-Adlrich |
Cat# ED2SS; CAS: 6381-92-6 |
| Glycine |
Fisher Scientific |
Cat# BP381-5 CAS: 56-40-6 |
| Sodium dodecyl sulfate |
Sigma-Aldrich |
Cat# 436143, CAS: 151-21-3 |
| Normal Goat Serum |
Vector Laboratories |
Cat# S-1000-20 |
| Neurobasal Medium |
Gibco |
Cat# 21103049 |
| Neurobasal Plus Medium |
Gibco |
Cat# A3582901 |
| B-27 Supplement |
Gibco |
Cat# 17504044 |
| B-27 Plus Supplement |
Gibco |
Cat# A3582801 |
| Glutamax Supplement |
Gibco |
Cat# 35050061 |
| Penicillin-Streptomycin |
Gibco |
Cat# 15070063 |
| Fetal Bovine Serum |
HyClone |
Cat# SH30070.03 |
| Poly-L-lysine hydrobromide |
Sigma-Aldrich |
Cat# P2636 |
| Paraformaldehyde (16%) |
Electron Microscopy Sciences |
Cat# 15711 |
| Tween 20 |
Fisher Scientific |
Cat# BP337-500; CAS: 9005-64-5 |
| Lipofectamine 2000 |
Invitrogen |
Cat# 11668019 |
| SR 95531 hydrobromide |
Tocris |
Cat# 1262 |
| Tetrodotoxin citrate |
Tocris |
Cat# 1069 |
| D-AP5 |
Tocris |
Cat# 0106 |
| MK-801 maleate |
Tocris |
Cat# 0924 |
|
| Experimental models: Cell lines |
|
| HEK293T |
ATCC |
Cat # CRL-3216 |
|
| Deposited data |
|
| Raw Mass Spectrometry Data Files |
This paper |
ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD041635 |
|
| Experimental models: Organisms/strains |
|
| Mouse: Btbd11 (rederived from frozen sperm) |
European Mouse Mutant Archive |
Btbd11tm1a(EUCOMM)Wtsi
|
| Mouse: vGAT-cre |
The Jackson Laboratory |
Strain #:028862 |
| Mouse: CaMKII-cre |
The Jackson Laboratory |
Strain #:005359 |
| Mouse: Psd95-GFP-ENABLED v2 |
From Haining Zhong, OHSU |
N/A |
| Rat: Sprague-Dawley (timed pregnant E18) |
Envigo |
Order code: 002-US |
|
| Oligonucleotides |
|
| Guide sequence to target the N terminus |
This paper |
N/A |
| of Btbd11: 5-ACGGCGGCTGCAGCATGAAG-3’ |
|
|
|
| Recombinant DNA |
|
| pAAV-S5E2-dTom-nlsdTom |
Addgene |
Cat# 135630 |
| pAAV-mDlx-GFP-Fishell-1 |
Addgene |
Cat# 83900 |
| pAAV-mDIx-Azurite |
This paper |
N/A |
| pORANGE Cloning template vector |
Addgene |
Cat# 131471 |
| Btbd11 (Myc-DDK-tagged) - Mouse BTB (POZ) domain containing 11 (Btbd11), transcript variant 1 |
Origene |
Cat# MR217199 |
| pCAG-Azurite |
(Fangetal., 2021)57
|
N/A |
| pCAG-GFP-Btbd11 |
This paper |
N/A |
| pCAG-mCherry-Btbd11 |
This paper |
N/A |
| pCAG-GFP-5xANK BTB (truncation) |
This paper |
N/A |
| pCAG-mCherry-5xANK BTB (truncation) |
This paper |
N/A |
| pCAG-GFP-Btbd11 Δ PBM |
This paper |
N/A |
| pCIS-GST-Btbd11 |
This paper |
N/A |
| pCIS-GST-Btbd11 Δ PBM |
This paper |
N/A |
| pDBLeu-Btbd11-C-terminus |
This paper |
N/A |
| pDBLeu-Btbd11-C-terminus Δ PBM |
This paper |
N/A |
| pDBLeu-Btbd11 (BTB domain only) |
This paper |
N/A |
| pPC86-PSD95 PDZ1 |
(Kim etal., 1998)58
|
N/A |
| pPC86-PSD95 PDZ2 |
(Kim etal., 1998)58
|
N/A |
| pPC86-PSD95 PDZ3 |
(Kim etal., 1998)58
|
N/A |
| pPC86-PSD95 PDZ1,2 |
(Kim etal., 1998)58
|
N/A |
| pPC86-PSD95 PDZ2,3 |
(Kim etal., 1998)58
|
N/A |
| pPC86-PSD95 PDZ1,2,3 |
(Kim etal., 1998)58
|
N/A |
| pCMV-Psd-95-mCherry |
(Araki et al., 2020)59
|
N/A |
| pCMV-Psd-95-mCherry Δ PDZ1,2 |
This paper |
N/A |
| pCMV-Psd-95-mCherry Δ PDZ3 |
This paper |
N/A |
| pCMV-Psd-95-mCherry Δ PDZ1,2,3 |
This paper |
N/A |
|
| Software and algorithms |
|
| LI-COR Image Studio |
LI-COR Biosciences |
N/A |
| ANY-maze behavior tracking software |
Stoelting |
N/A |
| ImageJ |
(Schneider et al. 2012)60
|
https://imagej.nih.gov/ij/
|
| IMARIS |
Oxford Instruments |
Version 9 |
| Illustrator |
Adobe |
Version 2023 |
| Prism |
Graphpad |
Version 9 |
| MATLAB |
Mathworks |
https://www.mathworks.com/
|
| Python 3.7 |
Jupyter notebooks |
Ver 3.7 |
|
| Other |
|
| Nanodrop One C |
Thermo Fisher |
Cat# 840–317500 |
| LSM 880 microscope |
Zeiss |
N/A |
