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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Hepatol. 2023 Jun 20;79(5):1214–1225. doi: 10.1016/j.jhep.2023.05.043

Fig. 6. Functional GARP is present on human HSCs and upregulated in the liver of fibrosis patients.

Fig. 6.

(A) Flow cytometry analysis of GARP expression on the surface of human primary HSCs after 7 days culture. (B) FACS analysis of GARP expression on the surface of human HSCs treated with or without scramble siRNA or GARP siRNA. (C) TGF-β activation by human HSCs treated with or without scramble siRNA or GARP siRNA. Data are presented as mean ± SEM. (D) Level of GARP mRNA expression in liver biopsies of patients with NASH (left), HBV-induced advanced fibrosis (middle), or HCV-induced cirrhosis (right) compared to corresponding non-fibrosis or healthy individuals. Solid black line in the violin plots represents median. The upper and lower dotted black lines represent 75th and 25th percentile. Each data point indicates one well in C or one patient in D. One-way ANOVA in C and unpaired t-test in D were used to compare two groups. ***p<0.001; ****p<0.001;NS, not significant.