KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Bacterial and virus strains | ||
|
| ||
| S. aureus USA300 LAC/MRSA WT | Boles et al.66 | AH1263 |
| S. aureus USA300 LAC Δspm/MRSA Δspm | This study | AH6121 |
| S. aureus USA300 LAC Δspm::spm/MRSA Δspm::spm | This study | AH6080 |
| S. aureus USA 300 LAC fur::tet | This study | AH4449 |
| S. aureus 8325-4 fur::tet | Horsburgh et al.67 | AH2367 |
| P. aeruginosa PAO1 | Holloway et al.68 | AH5602 |
| P. aeruginosa PAO1 ΔpqsA | Toyofuku et al.50 | AH5136 |
| P. aeruginosa PAO1 ΔpvdI | This study | AH6075 |
| P. aeruginosa PAO1 ΔpchF | This study | AH6197 |
| P. aeruginosa PAO1 ΔpvdI ΔpchF | This study | AH6076 |
| P. aeruginosa PAO1 Δpch | This study | AH6148 |
| E. coli DC10b | Monk et al.69 | AH2811 |
| E. coli Top10 | Invitrogen | N/A |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Methanol | Fisher Chemical | A456-212 |
| Ethyl Acetate | J.T. Baker | 02-002-839 |
| Acetonitrile | Fisher Chemical | A955-212 |
| Formic Acid | Fisher Chemical | A117-50 |
| 2-Cyano phenol | Chem-Impex Inc. | 26803 |
| D-cysteine hydrochloride hydrate | Sigma-Aldrich | C8005 |
| TMS-diazomethane | Sigma-Aldrich | 527254 |
| Formaldehyde (37% by weight in water) | Sigma-Aldrich | 252549 |
| N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide HCl | Sigma-Aldrich | 17750 |
| Hydroxy benzotriazole | Sigma-Aldrich | 157260 |
| Lithium aluminum hydride | Sigma-Aldrich | 593702 |
| Hexadecyltrimethylammonium bromide (HDTMA) |
Sigma-Aldrich | H9151 |
| 2′,7′-dichlorofluorescin diacetate | Sigma-Aldrich | D6883-250MG |
| 2,2′-Bipyridine | Thermo-Scientific Chemicals | A15782-14 |
| Chrome azurole S | Honeywell | 72687-25G |
|
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| Critical commercial assays | ||
|
| ||
| QIAprep Spin Miniprep Kit | Qiagen | 27104 |
| QIAquick Gel Extraction Kit | Qiagen | 28704 |
| QIAquick PCR Purification Kit | Qiagen | 28104 |
| iTaq Universal SYBR Green Supermix | Bio-Rad | 1725121 |
| iScript RT Supermix | Bio-Rad | 1708841 |
|
| ||
| Deposited data | ||
|
| ||
| mass spectrometry data | This study | MassIVE: MSV000089230, MassIVE: MSV000089231, MassIVE: MSV000089233, MassIVE: MSV000089234 |
|
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| Experimental models: Organisms/strains | ||
|
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| Mouse: C57BL/6J | Jackson Laboratories | 000664 |
|
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| Oligonucleotides | ||
|
| ||
| See Table S1 | N/A | |
|
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| Recombinant DNA | ||
|
| ||
| pJB38; Mutation generation vector | Bose et al.70 | pJB38 |
| pEX2G; Mutation generation vector | Rietsch et al.71 | pEX2G |
| pHC48; S. aureus constitutive dsRed expression vector | Ibberson et al.72 | pHC48 |
| pJBspm; pJB38-spm; construct for unmarked deletion of spm | This study | pJBspm |
| pJBspmKI; pJB38-spmKI; construct to repair Δspm mutant strain; water mark: NcoI restriction site |
This study | pJBspmKI |
| pJBfhuD1; pJB38-fhuD1; construct for unmarked deletion of fhuD1 | This study | pJBfhuD1 |
| pJBfhuD2; pJB38-fhuD2; construct for unmarked deletion of fhuD2 | This study | pJBfhuD2 |
| pJBfhuG; pJB38-fhuG; construct for unmarked deletion of fhuG | This study | pJBfhuG |
| pEXpch; pEX2G-pch; construct for unmarked deletion of the entire pyochelin biosynthesis cluster | This study | pEXpch |
| pEXpchF; pEX2G-pchF; construct for unmarked deletion of pchF | This study | pEXpchF |
| pEXpvdI; pEX2G-pvdI; construct for unmarked deletion of pvdI | This study | pEXpvdI |
| pHCspmprom; pHC48-spmprom, promoter region of the spm/fhuD1 operon with dsRed on the pHC48 backbone. | This study | pHCspmprom |
|
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| Software and algorithms | ||
|
| ||
| Prism, V9.4.1 | GraphPad Software | https://graphpad.com/scientificsoftware/prism |
| CFX Manager, V3.1 | Bio-RAD | https://www.bio-rad.com/en-us/sku/1845000-cfx-manager-software?ID=1845000 |
| MZmine, V2.53 | Pluskal et al.73 | http://mzmine.github.io |
| GNPS V30 | Wang et al.51 | https://gnps.ucsd.edu/ProteoSAFe/static/gnps-splash.jsp |
| FlexImaging, V4.1 | Bruker Daltonics | https://www.bruker.com |
| DataAnalysis, V4.2 | Bruker Daltonics | https://www.bruker.com |
| Cytoscape, V3.8.2 | Cytoscape Consortium | https://cytoscape.org |