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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Dev Dyn. 2023 May 15;252(10):1303–1315. doi: 10.1002/dvdy.597

Figure 2: Differential expression analysis of genes in WT, Six1-het and Six1-null mandibular arches.

Figure 2:

(A) Heatmap with K-means cluster analysis of averaged, z-score scaled, FPKM values for gene expression in each genotype. Yellow bars mark cluster number. Grey boxes list gene ontology terms represented in each cluster. (B) Quantitative-PCR (qPCR) validation of expression pattern of select genes from cluster 1 (Bgn), 5 (Frzb), 6 (Lum, Tubb3), 7 (Alx1, Fgf9) in WT, Six1-het (HET) and Six1-null (NULL) arches. *P<0.05, ** P< 0.01, *** P<0.001, **** P<0.0001, ns: not significant. n=3. Actb was used as housekeeping gene.