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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Dev Dyn. 2023 May 15;252(10):1303–1315. doi: 10.1002/dvdy.597

Figure 4: Regulation of WNT signaling by SIX1.

Figure 4:

(A-C) Whole-mount ISH for Frzb in E10.5 WT (A), Six1-het (HET) (B), and Six1-null (NULL) (C) mouse embryos. Ventral view. The heart was removed to aid visualization. 1. Mandibular arch. Loss of Six1 disrupts expression of Frzb in the mandibular arch (arrow). (D-E) Luciferase activity of the TOP-Flash reporter vector is significantly decreased in MC3T3-E1 (D) and O9-1 (E) cells after Six1 overexpression indicating that SIX1 represses canonical WNT-specific transcriptional activity. ***P<0.001, ****P<0.0001. n=3.