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[Preprint]. 2023 Oct 13:2023.02.24.529909. [Version 3] doi: 10.1101/2023.02.24.529909

Figure 2. Changes in the histone H3K4me2 modification in germline-expressed genes.

Figure 2.

H3K4me2 signal around gene promoters (−200–+500 bp) with transcripts enriched in specific germline cell types. Transcript expression was derived from FCA snRNA-seq clustering [Raz et al, 2023].

(A) Expression (snRNA-seq) in wildtype testes and H3K4me2 enrichment in bam mutant, in aly mutant, and in wildtype testes in germline stages. Z-scores for H3K4me2 signal were calculated between the three genotypes. Notable germline-enriched genes are indicated, including those expressed in GSCs, in meiosis, or for cell division (black), linked to the Y chromosome (purple), or expressed post-meiotically (orange).

(B) Selected examples of promoters with germline-enriched expression. Expression z-scores from FCA snRNA-seq across 18 germline clusters (Raz et al. 2023) and H3K4me2 enrichment in bam mutant, in aly mutant, and in wildtype testes.

(C) Distribution of H3K4me2 around promoters with germline-enriched expression. The testis somatic category comprises the top tercile of promoters with somatic cell-type expression in snRNA-seq data (Raz et al. 2023). Only promoters with no promoter of a second gene within 1 kb upstream are shown.