KEY RESOURCES TABLE
| REAGENT or RESOURCE SOURCE IDENTIFIER | ||
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Leu-Enkephalin | Abcam | Cat # ab150346 |
| Rabbit polyclonal anti-c-Fos | EnCor | Cat # RPCA-c-FOS |
| Chicken polyclonal anti-GFP | Aves | Cat # GFP-1020 |
| Mouse monoclonal anti-NeuN | Millipore | Cat # MAB377 |
| Rabbit polyclonal anti-RFP | Rockland | Cat # 600-401-379 |
| Goat polyclonal anti-Sox9 | RnD | Cat # AF3075 |
| Rabbit polyclonal anti-Urocortin-3 | Cosmo Bio | Cat # YII-Y364-EX |
| Rabbit polyclonal anti-ZIC | Segal Lab, DFCI57 | N/A |
| Donkey polyclonal anti-goat Alexa 488 | Thermo Fisher | Cat # A11055 |
| Donkey polyclonal anti-rabbit 546 | Thermo Fisher | Cat # A10040 |
| Goat polyclonal anti-chicken Alexa 488 | Thermo Fisher | Cat # A11039 |
| Goat polyclonal anti-mouse Alexa 488 | Thermo Fisher | Cat # A11001 |
| Goat polyclonal anti-rabbit Alexa 488 | Thermo Fisher | Cat # A11008 |
| Goat polyclonal anti-rabbit Alexa 546 | Thermo Fisher | Cat # A11010 |
| Goat polyclonal anti-rat Alexa 647 | Thermo Fisher | Cat # A21247 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (Ai 14) | Jackson Laboratory | Stock no. 007914 |
| Mouse: C57BL/6J-Tg(Nkx2-1-cre)2Sand/J (Nkx2.1-Cre) | Jackson Laboratory | Stock no. 008661 |
| Mouse: B6.129-Prdm16tm11Brsp/J (Prdm16f/f) | Jackson Laboratory | Stock no. 024992 |
| Bacterial and Virus Strains | ||
| AAV8-hSyn-FLEX-TVA-P2A-eGFP-2A-oG | Salk Institute | Addgene 85225 |
| EnvA G-Deleted Rabies-mCherry | Salk Institute | Addgene 32636 |
| Oligonucleotides | ||
| RNAscope probe for Crhr2 | ACD | Cat # 413201 |
| Software and Algorithms | ||
| Fiji 2.9.0/1.54e | Schindelin et al. 201258 | https://fiji.sc |
| Prism 9 | GraphPad | |