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[Preprint]. 2023 Oct 10:2023.09.29.560131. [Version 2] doi: 10.1101/2023.09.29.560131

Figure 1: Overview of experimental workflow.

Figure 1:

F0 generation females (6–8 weeks of age) were exposed to either 32ppm of Pb via drinking water or 5mg/kg-day of DEHP via food, beginning two weeks prior to mating using virgin males (8–10 weeks of age). Exposure to Pb or DEHP or control continued through gestation and weaning, when F1 mice were removed from the dams and placed on control water or chow. At 5 months of age, F1 mice were sacrificed, and genomic DNA was extracted from blood, liver, and cortex tissues. DNA was used to prepare libraries for Whole Genome Bisulfite Sequencing (WGBS). Following initial data processing, Differentially Methylated Regions (DMRs) were called using MethylSig and metilene.