Skip to main content
[Preprint]. 2023 Oct 10:2023.10.10.561687. [Version 1] doi: 10.1101/2023.10.10.561687

Figure 1. RAP2 mediates Hippo regulation by environmental signals and stresses.

Figure 1.

A. Wildtype (WT) and RAP2A/B/C triple knockout (tKO) cells were treated with cAMP inducers IBMX and FSK for 30 and 60 minutes. Then the cell lysates were collected for analyses of LATS phosphorylation at hydrophobic motif (HM) and YAP phosphorylation at Serine 127. B. WT and RAP2A/B/C tKO cells were serum starved, by replacing 10% FBS DMEM with serum-free DMEM (−FBS), for 30 and 60 minutes. C. WT and RAP2A/B/C tKO cells were treated with the Rho inhibitor C3 for 1.0 and 3.0 hours for analyses of the role of RAP2 in Hippo regulation by Rho GTPase. D. WT and RAP2A/B/C tKO cells were treated with an energy stress-inducer 2-deoxy-D-glucose (2-DG) for 30 and 60 minutes. E. WT and RAP2A/B/C tKO cells were treated with an energy stress-inducer sorbitol for 30 and 60 minutes. F. A diagram of a working model illustrating the role of RAP2 GTPase in the Hippo signaling regulation by environmental signals and stress.