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[Preprint]. 2023 Oct 10:2023.10.10.561687. [Version 1] doi: 10.1101/2023.10.10.561687

Figure 7. RAP2 GTPase is involved in Hippo signaling regulation by tubulin cytoskeleton dynamics.

Figure 7.

A. WT, RAP2A/B/C tKO, and MAP4K4/6/7 tKO HEK293A cells were treated with Taxol (3 μM) for 120 minutes. B & C. Representative bright filed images (B) and cell viability MTS test (C) showed cell survival of WT and RAP2A/B/C tKO MCF10AT cells treated with 50 nM of Taxol for 24 hours. For the MTS assay, a Two-way ANOVA and Tukey’s multiple comparison test was applied. ns, not significant; ****, p<0.0001. Scale bar, 300 μm. D. Transwell migration assay on WT and RAP2A/B/C tKO MCF10AT cells treated with 50 nM of Taxol for 24 hours. Scale bar, 100 μm. E. Quantification of cell migration assay. Student t-test was performed to determine the p value.