TABLE 1.
Lactacystin inhibits development of sporozoites into EEF
| Expt | Inhibitor | Concn (μM) | No. of EEFa | % Inhibition |
|---|---|---|---|---|
| 1b | None | 448 ± 25 | ||
| Lactacystin | 1 | 109 ± 10 | 75 | |
| 3 | 5 ± 2 | 99 | ||
| 9 | 0 | 100 | ||
| 2c | None | 291 ± 5 | ||
| Lactacystin | 1 | 156 ± 6 | 46 | |
| 3 | 34 ± 5 | 88 | ||
| 9 | 0 | 100 | ||
| clasto-Lactacystin dihydroxy acid | 9 | 280 ± 32 | 6 | |
| 3d | None | 816 ± 9 | ||
| Lactacystin | 1 | 574 ± 13 | 29 | |
| 3 | 458 ± 17 | 43 | ||
| 9 | 408 ± 3 | 50 |
a
Number of EEF per 100 high-powered fields.
b
Inhibitor present for the first 3 h of the experiment.
c
Parasites were preincubated with inhibitor for 1 h, washed, and then plated on cells.
d
Inhibitor was added 24 h after parasites were plated on cells. The EEF in the lactacystin-treated wells were smaller than those in the control wells.