TABLE 3.
Lactacystin inhibits sporozoite infectivity in vivo
| No. of sporozoites injected | Inhibitora | No. of mice positive for parasites/no. of mice injectedb on day:
|
||
|---|---|---|---|---|
| 3 | 4 | 5 | ||
| 100,000 | None | 1/1 | ||
| Lactacystin | 2/2 | |||
| 10,000 | None | 2/2 | ||
| Lactacystin | 0/3 | 3/3 | ||
| 1,000 | None | 3/3 | ||
| Lactacystin | 0/3 | 3/3 | ||
| 100 | None | 0/3 | 3/3 | |
| Lactacystin | 0/3 | 0/3 | 2/3c | |
a
Sporozoites were preincubated in medium with 3 μM lactacystin or without lactacystin for 1 h and then injected into mice i.v.
b
On days 3 to 5 postinjection blood smears were taken from mice and analyzed for the presence of parasites.
c
The mouse that was negative on day 5 remained negative for the next 7 days.