Figure 3. Partial normalization of network modularity after treatment with R121919 in alcoholdependent mice.

(A-F) Network modules obtained through hierarchical clustering of regions based on their correlation and cutting the dendrogram at half-height (dotted line, n=number of clusters) in correlation heatmaps from blue (= anticorrelated) to red (= correlated): for control mice treated with saline (A), R121919 (B), or naltrexone (C) and alcohol-dependent mice in withdrawal treated with saline (D), R121919 (E), or naltrexone (F); sidebar indicates the anatomical group the region belongs to: cortical plate (blue), cortical subplate (light blue), striatum (yellow), pallidum (orange), thalamus (red), hypothalamus (pink), midbrain (green), hindbrain (gray). The specific order of the regions in these hierarchically clustered heatmaps can be found in the supplementary information (Supp. Table 2). (G-H) Number of modules as a function of tree cut for control animals (G) and alcohol-dependent animals (H). (I-J) Network centrality measures identifying the CEA (red dot and arrow) as a hub region in the dependent, saline-treated network with high degree (I) and betweenness (J); *p<0.001 compared to non-dependent and ^#p<0.001 compared to dependent saline-treated animals).