KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| (Flow Cytometry –Cell Surface Staining) | ||
| B220 | BioLegend | 103206 |
| CD3 | BioLegend | 100218 |
| CD3 | BioLegend | 100205 |
| CD4 | BioLegend | 100430 |
| CD45 | BioLegend | 103147 |
| CD8a | BioLegend | 100722 |
| CD8a | BioLegend | 100712 |
| CD11b | BioLegend | 101226 |
| CD11c | BioLegend | 117308 |
| CD16/32 | BioLegend | 156604 |
| F4/80 | BioLegend | 123130 |
| IFNγR1(CD119) | ThermoFisher | A16396 |
| IFNγR1(CD119) | BioLegend | 308704 |
| IFNγR1(CD119) | BioLegend | 308606 |
| Ly6C | BioLegend | 127628 |
| Ly6G | BioLegend | 128035 |
| MHCII | BioLegend | 107636 |
| NK1.1 | BioLegend | 108725 |
| PD-1 | BioLegend | 135216 |
| PD-L1(CD274) | BioLegend | 124308 |
| H-2Kd/H-2Dd | BioLegend | 114708 |
| IgG2a,k isotype Ctrl | BioLegend | 400212 |
| Human CD119 | BioLegend | 308606 |
| Antibodies | ||
| (Flow Cytometry –Intracellular Staining) | ||
| Foxp3 | BioLegend | 11-5773-82 |
| Foxp3 | ThermoFisher | 69-5773-82 |
| GzmB | ThermoFisher | 12-8898-82 |
| GzmB | ThermoFisher | 17-8898-82 |
| IFNγ | BioLegend | 505806 |
| IFNγR1(CD119) | Abcam | ab95673 |
| PD-L1(CD274) | BioLegend | 124308 |
| Antibodies | ||
| (Western Blot-Primary Antibodies) | ||
| ARF6 | Cell Signaling Technology | 5740s |
| α-tubulin | Cell Signaling Technology | 3873s |
| ARF1 | Invitrogen | PA1–127 |
| GAPDH | Cell Signaling Technology | 5174 |
| IFNγR1 | Abcam | ab280353 |
| IFNγR1 | Santa Cruz Biotechnology | SC-28363 |
| IDO | Cell Signaling Technology | 86630S |
| IDO | Cell Signaling Technology | 5185S |
| CD80 | Cell Signaling Technology | 54521S |
| JAK1 | Cell Signaling Technology | 3344S |
| p-JAK1 (Tyr1034/1035) | Cell Signaling Technology | 74129S |
| Lamp1 | Cell Signaling Technology | 9091S |
| PD-L1 | Cell Signaling Technology | 60475S |
| PD-L1 | Cell Signaling Technology | 13684S |
| STAT1 | Cell Signaling Technology | 14994S |
| p-STAT1(Tyr701) | Cell Signaling Technology | 7649S |
| LSECtin | Abcam | ab181196 |
| Galectin-3/LGALS3 | Cell Signaling Technology | 12733S |
| Galectin-3/LGALS3 | Cell Signaling Technology | 87985S |
| Antibodies | ||
| (Western Blot-Secondary Antibodies) | ||
| IgG | Jackson ImmunoReseach | 715-035-152 |
| IgG | Jackson ImmunoReseach | 711-035-152 |
| Chemical Compounds | ||
| Recombinant Murine IFNy | PeproTech | 315–05 |
| Recombinant Human IFNy | PeproTech | 300–02 |
| Bafilomycin A1 | Sigma-Aldrich | SML1661 |
| MG132 | MedChemExpress | HY-13259 |
| QS11 | Tocris Bioscience | 3324 |
| Oligonucleotides (qRT-PCR) | ||
|
Ifnγr1 Forward sequence 5’-CTTGAACCCTGTCGTATGCTGG-3’ |
This study | n/a |
|
Ifnγr1 Reverse sequence 5’ -TTGGTGCAGGAATCAGTCCAGG-3’ |
This study | n/a |
|
Gapdh Forward sequence 5’-AGGTCGGTGTGAACGGATTTG-3’ |
This study | n/a |
|
Gapdh Reverse sequence 5’-TGTAGACCATGTAGTTGAGGTCA-3’ |
This study | n/a |
|
Pd-l1(Cd274) Forward sequence 5’-GCTCCAAAGGACTTGTACGTG-3’ |
This study | n/a |
|
Pd-l1(Cd274) Reverse sequence 5’-TGATCTGAAGGGCAGCATTTC-3’ |
This study | n/a |
|
Ido1 Forward sequence 5’-GGGCTTCTTCCTCGTCTCTC-3’ |
This study | n/a |
|
Ido1 Reverse sequence 5’-TGGATACAGTGGGGATTGCT-3’ |
This study | n/a |
| Oligonucleotides (PCR) | ||
|
Arf6 Forward sequence 5’- TGAGGCATACACCATTATTGCTCC −3’ |
This study | n/a |
|
Arf6 Reverse sequence 5’- GTAATAGCAGTGTAATGTTCCAGTTG-3’ |
This study | n/a |