| Antibodies |
| rabbit α-RFP |
Rockland |
Cat#600-401-379; RRID: AB_2209751 |
| donkey α-rabbit Alexa Fluor 555 |
Invitrogen |
Cat#A-31572; RRID: AB_162543 |
| Bacterial and virus strains |
| AAV2-hSyn-DIO-mCherry |
Addgene |
50459-AAV2 |
| AAV2-hSyn-DIO-hM3Dq-mCherry |
Addgene |
44361-AAV2 |
| AAVr-Ef1A-Cre |
Addgene |
55636-AAVrg |
| AAVr.hSyn.HI.eGFP-Cre.WPRE.SV40 |
Addgene |
105540-AAVrg |
| Chemicals, peptides, and recombinant proteins |
| Compound 48/80 |
Sigma-Aldrich |
Cat#C2313; CAS: 848035-21-2 |
| Chloroquine Diphosphate Salt |
Sigma-Aldrich |
Cat#C6628; CAS: 50-63-5 |
| FAST DiI™ oil |
Invitrogen |
Cat#D3899 |
| Octreotide |
Tocris Bioscience |
Cat#1818; CAS: 83150-76-9 |
| GRP |
Tocris Bioscience |
Cat#1789; CAS: 93755-85-2 |
| Substance P |
Sigma-Aldrich |
S6883; CAS: 137348-11-9 |
| Taltirelin (Talt) |
Adooq Bioscience |
Cat#A18250; CAS: 103300-74-9 |
| Oxytocin (Oxy) |
Sigma-Aldrich |
O4375; CAS: 50-56-6 |
| Oxotremorine (Oxo) |
Tocris Bioscience |
Cat#1067; CAS: 3854-04-4 |
| Cholecystokinin Octapeptide, sulfated (CCK) |
Tocris Bioscience |
Cat#11661 |
| Neurokinin B (NKB) |
Tocris Bioscience |
Cat#1908; CAS: 87096-84-2 |
| Tetrodotoxin citrate (TTX) |
Abcam |
Cat#ab120055; CAS: 18660-81-6 |
| GR, 73632 |
Tocris Bioscience |
Cat#1669; CAS: 133156-06-6 |
| Nalfurafine Hydrochloride |
Adooq Bioscience |
Cat#A12579; CAS: 152658-17-8 |
| 2-Aminoethoxydiphenyl borate (2-APB) |
Tocris Bioscience |
Cat#1224; CAS: 524-95-8 |
| Cyclopiazonic acid (CPA) |
Tocris Bioscience |
Cat#1235; CAS: 18172-33-3 |
| Critical commercial assays |
| RNAscope™ Probe- Mm-Grpr |
ACDbio |
Cat#317871 |
| RNAscope™ Probe- Mm-Tacr1 |
ACDbio |
Cat#428781 |
| RNAscope™ Probe- Cre |
ACDbio |
Cat#474001 |
| RNAscope™ Probe- Mm-Slc17a6 |
ACDbio |
Cat#456751 |
| RNAscope™ Probe- Mm-Tac1 |
ACDbio |
Cat#410351 |
| RNAscope™ Probe- Mm-Grp |
ACDbio |
Cat#317861 |
| RNAscope™ Probe- Mm-Oprk1 |
ACDbio |
Cat#316111 |
| RNAscope™ Probe- EGFP |
ACDbio |
Cat#400281 |
| Experimental models: Organisms/strains |
| Mouse: C57BL6/J
|
Charles River |
Cat: 027 |
| Mouse: Vglut2-Cre
|
Vong et al.67
|
Jax Stock #016963 |
| Mouse: Ai96 (RCL-GCaMP6s)
|
Madisen et al.68
|
Jax Stock #028866 |
| Mouse: Snap25-2A-GCaMP6s-D
|
Madisen et al.68
|
Jax Stock #025111 |
| Mouse: Grpr-Cre
|
Munanairi et al.38
|
Jax Stock #036668 |
| Mouse: Oprk1-Cre
|
Cai et al.69
|
Jax Stock #035045 |
| Software and algorithms |
| PRISM 9.0 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|
| FIJI |
ImageJ |
https://www.nature.com/articles/nmeth.2019
|
| suite2p |
v0.10.0, HHMI Janelia |
https://www.suite2p.org/
|
| R |
R Core Team (2021)70
|
https://www.r-project.org/
|
| Python |
Python 3.10.7 |
https://www.python.org/
|
| EulerAPE |
Micallef and Rodgers66
|
https://www.eulerdiagrams.org/eulerAPE/
|
