Figure 2:

Transwell synovial cell migration assay. Schematic of the experimental set up (A). Resident synovial macrophage markers TREM-2+, OPG+, CX3CR1+, ZO-1+ and F11R+ were evaluated within the migratory monocyte/macrophage population (Alive/CD56−/CD3−/CD11c−/CD20−/CD14+/CD11b+) in a representative patient treated with 250 ng/mL of MCP-1 (B). tSNE plots of the same patient’s migratory myeloid cells demonstrating ZO-1 has the most delineation from other markers (C).