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[Preprint]. 2023 Oct 4:2023.10.03.560719. [Version 1] doi: 10.1101/2023.10.03.560719

Extended Data Figure 2. Disruption of LIG1-UHRF1 binding destabilizes LIG1 in chromatin.

Extended Data Figure 2.

a Protein levels in LIG1-depleted U2OS cells..

b Lengths of IdU labeled replication tracts in LIG1-depleted U2OS cells.

c Protein levels in UHRF1-depleted U2OS cells.

d Lengths of IdU labeled replication tracts in UHRF1-depleted U2OS cells.

e Co-IP of endogenous UHRF1 with FLAG-HA-tagged WT LIG1 in U2OS cells stably expressing the protein treated with DMSO, UNC0638 or UNC0642 (4 µM) for 54 h.

f Protein levels of LIG1, UHRF1, PCNA and FEN1 in the whole cell as well as soluble and chromatin fractions of 293T cells transiently transfected with LIG1 WT, K126R and K126A cDNA constructs.

Data (b and d dots=200) are presented as mean ± standard deviations (SD). Statistical test is preformed using two-tailed unpaired Student’s t test and the exact p value is provided.