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[Preprint]. 2023 Oct 3:2023.10.03.560700. [Version 1] doi: 10.1101/2023.10.03.560700

Figure 1. Post-ischemic inactivation of endothelial PHD2 does not alter post-ischemic kidney injury.

Figure 1.

(A) Experimental scheme illustrates the timing of unilateral renal artery clamping, tamoxifen administration and analysis. (B) Representative images of H&E and Picro-Sirius red stained sections from day 14 post-ischemic kidneys of PHD2iEC mutants and their Cre− littermates. Right panels show tubular injury score (Top) and semi-quantitative analysis of Picro-Sirius red+ve area in the indicated geno-types. Scale bars indicate 100 μm and 200 μm for H&E and Picro-Sirius red images, respectively. (C) mRNA levels of Loxl2, Tgfb1 and Acta2 in IR and CTL kidneys from PHD2iEC mice and their Cre− controls at day 14 after unilateral IRI. All bars show mean ± SEM. For (B), unpaired t-test with Welch’s correction was used. For (C), statistics were determined using one-way ANOVA with Sidak correction for multiple comparisons. *, P <0.05; ns, not statistically significant. uIRI, unilateral ischemia reperfusion injury; CTL, contralateral; IR, kidney subjected to uIRI; Rel., relative.