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[Preprint]. 2023 Oct 3:2023.10.02.560521. [Version 1] doi: 10.1101/2023.10.02.560521

Figure 7. STIM2 deletion promotes cMyc stabilization and increases PPARɣ levels.

Figure 7.

(A) Representative western blot probed with anti- phospho-cMyc, cMyc, phospho-ERK, ERK, and GAPDH.

(B, C) Densitometric analysis of (B) phospho-cMyc and (C) phospho-ERK in HCT116, STIM1 KO #01, STIM2 KO #15, DKO #22 clone in HCT116 and DLD1, STIM1 KO #03, STIM2 KO #04, DKO #01 clones in DLD1 cells.

(D-G) PGC1α levels determined by western blot (D, F) blot probed with anti- PGC1α and GAPDH antibody and (E, G) densitometric analysis of PGC1α levels in HCT116, DLD1, STIM1 KO, STIM2 KO, and DKO clones of HCT116 and DLD1 cells.

(H, I) PPARɣ levels quantified using (H) western blot probed with anti-PPARɣ and GAPDH antibody, and (I) densitometric analysis of PPARɣ levels in HCT116, DLD1, STIM1 KO, STIM2 KO, and DKO clones of HCT116 and DLD1 cells.

All experiments were performed ≥three times with similar results. Statistical significance was calculated using paired t-test. *p<0.05, **p<0.01, ***p<0.001