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[Preprint]. 2023 Oct 3:2023.10.02.560521. [Version 1] doi: 10.1101/2023.10.02.560521

Figure 8. Loss of STIM2 activates the ATF4-dependent ER stress pathway.

Figure 8.

(A, B) Quantification of BiP protein levels using (A) western blot probed with anti-Bip and GAPDH antibody, and (B) densitometric analysis in HCT116, DLD1, STIM1 KO, STIM2 KO, and DKO clones of HCT116 and DLD1cells.

(C, D) Quantification of ATF4 protein levels using (C) western blot probed with anti-ATF4 and GAPDH antibody, and (D) densitometric analysis in HCT116, STIM1 KO, STIM2 KO, and DKO clones of HCT116.

(E, F) Quantification of IRE1α protein levels using (E) western blot probed with anti- IRE1α and GAPDH antibody, and (F) densitometric analysis in HCT116, STIM1 KO, STIM2 KO, and DKO clones of HCT116.

(G, H) Quantification of ATF6 protein levels using (G) western blot probed with anti- ATF6 and GAPDH antibody, and (H) densitometric analysis in HCT116, STIM1 KO, STIM2 KO, and DKO clones of HCT116.

(I, J) Quantification of phospho-eIF2α protein levels using (I) western blot probed with anti-phospho-eIF2α, eIF2α, and GAPDH antibody, and (J) densitometric analysis in HCT116, STIM1 KO, STIM2 KO, and DKO clones of HCT116.

(K, L) Quantification of phospho-PERK protein levels using (K) western blot probed with anti-phospho-PERK, PERK, and GAPDH antibody, and (L) densitometric analysis in HCT116, STIM1 KO, STIM2 KO, and DKO clones of HCT116.

All experiments were performed ≥three times with similar results. Statistical significance was calculated using paired t-test . *p<0.05, **p<0.01, ***p<0.001