Table 3.
The matrix effect can be quantitatively evaluated by comparing the concentration of the analyte in standard solution (A) to that of a post-extract spiked with the analyte at the same concentration (B).
| Compound | Calibration curve (A) | Spiked (B) | Matrix effect % |
|---|---|---|---|
| PGB2-d4 | 15.63 | 19.73 | −26.25±5.56 |
| LTB4-d4 | 10.08 | 9.83 | 2.45± 8.28 |
| 8,9-DiHETrE-d11 | 15.70 | 18.83 | −19.96 ± 2.21 |
| 9-HODE-d4 | 16.70 | 20.63 | −23.55±9.22 |
| 15(S)-HETE-d8 | 20.50 | 23.30 | −13.66±5.22 |
| 5-HETE-d8 | 42.00 | 56.97 | −35.63± 7.49 |
| 8,9-EET-d11 | 42.10 | 56.50 | −34.19± 1.87 |