Figure 4.

North American Airway Collaborative (NoAAC) cell atlas of idiopathic subglottic stenosis (iSGS). (A) Single cell RNA sequencing (scRNAseq) results of 7 iSGS airway scar samples consisting of 34,000 cells with unsupervised clustering analysis for cell subtype. (B) Tissue-specific expression of 20 candidate risk genes associated with proximal airway stenosis (average quantitative RNA expression denoted by color gradient, percentage of cells showing expression within a tissue type denoted by size of dot). (C) Percentage of genes significantly enriched in each tissue type. Genes were defined as enriched in a specific cell type if their expression in that cell type exceeded all others by >½ a standard deviation (34.1%). Genes without a cell type fulfilling enrichment criteria were categorized as “Ubiquitous”.