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. 2022 Dec 26;2022:0002. doi: 10.34133/bdr.0002

Table.

Yeast strains constructed in this study.

Strain Genotype Source
AJM7 CEN.PK2-Int16::CPR1-CYB5; ΔPERG20::PHXT1;Int9::tGES ~ ERG20WW; Int11::G8H; Int14::ISY2-GOR;Int4::TDC-IDI1; Int10::GPPS2-tHMGR; Int6::G8H;Int12::TDC-CYPADH2; Int17::SLS2-STR; Int19::LAMT-7DLGT; Int7::IO-7DLH; Int18::SGD-HYS; ΔOYE2::ZWF1-GAPN; ΔOYE3::ERG20WW-MLPLA; ΔARI1::SAM2; ΔADH6::SLS2-STR; Int5::GAL4 [12]
AJM7-ΔHYS AJM7-ΔHYS This study
AJM7A AJM7-ΔHYS-IntG20::HYS This study
CAT module
CAT0 AJM7-ΔHYS-IntL7::GS-SAT; IntL9::PAS This study
CAT2 CAT0-IntL10::GO-DPAS; IntL11::Redox2; IntL12::Redox1-CS This study
CAT3 CAT2-IntL5::GS This study
CAT4 CAT2-IntL5::GO This study
CAT5 CAT2-IntL5::Redox1 This study
CAT6 CAT2-IntL5::Redox2 This study
CAT7 CAT2-IntL5::SAT This study
CAT8 CAT2-IntL5::PAS This study
CAT9 CAT2-IntL5::DPAS This study
CAT10 CAT2-IntL5::CS This study
TAB module
TAB3 CAT0-IntL10::GO-DPAS; IntL11::Redox2; IntL12::Redox1-TS This study
TAB4 TAB3-IntL5::GS This study
TAB5 TAB3-IntL5::GO This study
TAB6 TAB3-IntL5::Redox1 This study
TAB7 TAB3-IntL5::Redox2 This study
TAB8 TAB3-IntL5::SAT This study
TAB9 TAB3-IntL5::PAS This study
TAB10 TAB3-IntL5::DPAS This study
TAB11A TAB3-IntL5::TS This study
VIN module
VIN1 TAB4-IntG16::16OMT-T16H2; IntG17::T3R-NMT This study
VIN3 VIN1-IntG22::T3O; IntG19::D4H-DAT This study
VIN5 VIN3-IntG24::TS This study
VIN7 VIN3-IntG24::GS This study
VIN8 VIN3-IntG24::DPAS This study
VIN10 VIN3-IntG24::TS; IntG25::GS This study
VIN11 VIN3-IntG24::TS; IntG25::DPAS This study
VIN12 VIN3-IntG24::GS; IntG15::DPAS This study
VIN13 VIN3-IntG21::TS; IntG24::GS; IntG15::DPAS This study

ERG20WW represents the ERG20F96W,N127W mutant.