Figure 6.

Regenerative effect of ASC-derived exosomes on cryoinjured corneal endothelium of rats. (A, B) Cells were stained with Ki67 as a proliferation marker. Scale bar: 50 µm. (C, D) Nestin expression was evaluated using immunofluorescence staining. Scale bar: 50 µm. (E) Mitochondrial membrane potential was assessed using JC-1 in rat corneal endothelium. Scale bar: 50 µm. One-half exosome = half dose of exosome group. (F) MitoSOX Red was used to evaluate the mitochondrial oxidation in rat corneal endothelium. Scale bar: 50 µm. Data represent mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.